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Polimorfismo enzimático em populações de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae) Zoologia
Aidar,Davi S.; Pagotto,Rubiane C.; Contel,Eucleia P.B..
Them aim scope of this study is to characterize the enzymatic polymorphism found in the Melipona quadrifasciata Lepeletier, 1936 populations from Ribeirão Preto, São Paulo and Espírito Santo, Brazil and its hybrids. Samples from each colony (about 52) were prepared for starch gel electrophoresis in order to investigate the genetic variation of the following enzimes: esterase (EST), isocitrate dehydrogenase (IDH), malic enzyme (ME), phosphoglucomutase (PGM), superoxide desmutase (SOD), α-glycerophosphate dehydrogenase (αPGD), malate dehydrogenase (MDH), leucine aminopeptidase (LAP), hexokinase (HK) and phosphoglucose isomerase (PGI). The analysis showed that LAP and HK did not show enzymatic activity and EST showed two alleles(est-sand and est-f) while all...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Melipona quadrifasciata anthidioides; Polymorphism; Electrophoresis; Enzyme; Stingless bees.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000200018
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Areas of natural occurrence of Melipona scutellaris Latreille, 1811 (Hymenoptera: Apidae) in the state of Bahia, Brazil Anais da ABC (AABC)
Alves,Rogério M.O.; Carvalho,Carlos A.L.; Souza,Bruno A.; Santos,Wyratan S..
The bee Melipona scutellaris is considered the reared meliponine species with the largest distribution in the North and Northeast regions of Brazil, with records from the state of Rio Grande do Norte down to the state of Bahia. Considering the importance of this species in the generation of income for family agriculture and in the preservation of areas with natural vegetation, this study aimed at providing knowledge on the distribution of natural colonies of M. scutellaris in the state of Bahia. Literature information, interviews with stinglessbee beekeepers, and expeditions were conducted to confirm the natural occurrence of the species. A total of 102 municipalities showed records for M. scutellaris, whose occurrence was observed in areas ranging from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stingless bee beekeeping; Uruçu bee; Stingless bees; Distribution.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300010
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Pythium insidiosum: inhibitory effects of propolis and geopropolis on hyphal growth BJM
Araújo,Maria José Abigail Mendes; Bosco,Sandra de Moraes Gimenes; Sforcin,José Maurício.
Abstract Propolis and geopropolis are resinous products of bees showing antimicrobial effects. There is no data concerning their action against Pythium insidiosum - the causative agent of pythiosis, a pyogranulomatous disease of the subcutaneous tissue that affects mostly horses, dogs and humans. Fragments of 15 isolates of P. insidiodum were incubated with propolis and geopropolis extracts and evaluated for up to seven days to detect the minimal fungicidal concentration (MFC). Propolis inhibited three isolates at 1.0 mg mL-1 after 24 h and all other isolates at 3.4 mg mL-1. Geopropolis led to more variable results, exerting predominantly a fungistatic action than a fungicidal one. Propolis was more efficient than geopropolis in inhibiting P. insidiosum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pythium insidiosum; Propolis; Geopropolis; Stingless bees; Africanized honeybees.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400863
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Occurrence of B chromosomes in Tetragonisca Latreille, 1811 (Hymenoptera, Apidae, Meliponini): a new contribution to the cytotaxonomy of the genus Genet. Mol. Biol.
Barth,Adriane; Fernandes,Anderson; Pompolo,Silvia das Graças; Costa,Marco Antônio.
Tetragonisca angustula and Tetragonisca fiebrigi have recently been listed as valid species. This study aimed to cytogenetically investigate both species, emphasizing the new registry of B chromosomes in the tribe Meliponini. We analyzed colonies of T. angustula and T. fiebrigi collected at Tangará da Serra, Mato Grosso, Brazil, through conventional Giemsa staining, C-banding, and base-specific fluorochrome staining (CMA3/DAPI). T. angustula showed 2n = 34 chromosomes in females and n = 17 in males, with karyotype formula 2K = 34A M. T. fiebrigi showed numeric variation, with chromosome number varying from 2n = 34 to 2n = 36 in females and from n = 17 to n=18in males, with karyotype formula 2K = 32A M+2A Mc and 2K = 32A M+2A Mc + 1 or 2 B-chromosomes. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stingless bees; Cytogenetics; Interspecific differentiation; B chromosomes.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100014
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Genetic variability in five populations of Partamona helleri (Hymenoptera, Apidae) from Minas Gerais State, Brazil Genet. Mol. Biol.
Borges,Andreia Arantes; Campos,Lucio Antônio de Oliveira; Salomão,Tânia Maria Fernandes; Tavares,Mara Garcia.
Partamona is a Neotropical genus of stingless bees that comprises 33 species distributed from Mexico to southern Brazil. These bees are well-adapted to anthropic environments and build their nests in several substrates. In this study, 66 colonies of Partamona helleri from five localities in the Brazilian state of Minas Gerais (São Miguel do Anta, Teixeiras, Porto Firme, Viçosa and Rio Vermelho) were analyzed using nine microsatellite loci in order to assess their genetic variability. Low levels of observed (Ho = 0.099-0.137) and expected (He = 0.128-0.145) heterozygosity were encountered and revealed discrete genetic differentiation among the populations (F ST =0.025). AMOVA further showed that most of the total genetic variation (94.24%) in P. helleri was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsatellites; Partamona helleri; Population genetics; Stingless bees.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400028
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Antibacterial activity of propolis produced by Frieseomelitta varia Ciência e Agrotecnologia
Campos,Viviane Aparecida Costa; Santos Júnior,Helvécio Martins dos; Oliveira,Denilson F; Carvalho,Hudson Walace Pereira de; Machado,Alan Rodrigues Teixeira; Tirelli,Aline Auxiliadora.
To contribute to the development of antibacterial products from propolis produced by native Brazilian bees, twenty-nine samples of propolis collected from hives in the state of Minas Gerais, Brazil, were screened for in vitro activity against Aeromonas hydrophila, Bacillus subtilis, Pseudomonas aeruginosa, and Staphylococcus aureus. Among the samples from native Brazilian bees, only that from Frieseomelitta varia (Lepeletier, 1836) inhibited in vitro bacterial growth. Consequently, this propolis underwent fractionation by chromatographic methods monitored through Agar-diffusion assays with these bacteria, which resulted in the isolation and identification of 3,5-diprenyl-4-hydroxycinnamic acid (artepillin C), which showed MIC of 62.5 and 250 µg/mL against...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artepillin C; Stingless bees; Antibiotic.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000600002
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Comparative study of wax glands in four Meliponini bees (Hymenoptera, Apidae) producing different quantities of wax Iheringia, Sér. Zool.
Cavalcante,Vagner Melo; Oliveira,Vagner Tadeu Paes de; Cruz-Landim,Carminda da.
The developmental degree of the wax glands was compared in four Meliponini bees, that produce different quantities of wax. The histological data and height average of the wax epithelium during the time in which the maximum production of wax is expected, are in accordance with the rates of wax produced by the species. In Lestrimelitta limao (Smith, 1863) a species which has cleptobiotic habits, and frequently rob wax from the attacked colonies, the height of wax epithelium was the lowest among the studied species. The cells seem to show an abnormal vacuolated cytoplasm, in the phase in which they would be producing wax.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stingless bees; Wax glands; Development; Wax production.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212000000200010
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Karyotypic description of four species of Trigona (Jurine, 1807) (Hymenoptera, Apidae, Meliponini) from the State of Mato Grosso, Brazil Genet. Mol. Biol.
Costa,Kleber França; Brito,Rute Magalhães; Miyazawa,Carlos Suetoshi.
The genus Trigona contains at least 31 species, but there have been few cytogenetic studies of this group. In this work, four species of Trigona (T. branneri, T. chanchamayoensis, T. hyalinata, and T. recursa) from the municipality of Cuiabá, in the State of Mato Grosso, Brazil, were studied. In all of the species, the females had 2n = 34 chromosomes and the males had n = 17. The C-banding patterns showed that the karyotypes of these species consisted mainly of acrocentric and pseudoacrocentric chromosomes. These cytogenetic findings should useful in future phylogenetic studies of this group.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytogenetics; Karyotype; Meliponini; Stingless bees; Trigona.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200010
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Note on glands present in meliponinae (Hymenoptera, Apidae) bees legs Zoologia
Cruz-Landim,Carminda da; Moraes,Regina Lúcia Morelli Silva de; Salles,Heliana Clara; Reginato,Rejane Daniele.
The present paper reports the presence of glandular structures in legs of some stingless bee species. The glands appear as: the epidermis transformation in a glandular epithelium as in basitarsus, an epithelial sac inside the segment as in the femur of queens or in the last tarsomere, as round glandular cells, scattered or forming groupments. The saculiform gland of femur is present only in queens, the other glands are present in males, queens and workers of the studied species, apparently without any type of polymorphism. This occurrence seems indicate that the function of these glands have not to do with the sociality or specific behavior of castes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hymenoptera; Stingless bees; Legs; Glands.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751998000100014
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Honey quality of Melipona sp. bees in Acre, Brazil Acta Agron. (Palmira)
Damasceno do Vale,Marcus Augusto; Gomes,Fábio Augusto; Cunha dos Santos,Betina Raquel; Batista Ferreira,Josimar.
Abstract Honey from stingless bees (Melipona sp.) is a nutritious and medicinal product economically valued in the informal Brazilian market, driven by the growing demand for natural products; however, its physicochemical characteristics are still unknown. Thus, the aim of this study is to evaluate the physicochemical profile of the stingless bee honey produced in Cruzeiro do Sul, Acre. Honey samples were analyzed following the methodology recommended by Ministério da Agricultura, Pecuária e Abastecimento; the following parameters were established: moisture, total sugars, reducing sugars, apparent sucrose, ash, crude protein, diastase activity, Brix degrees, free acidity, lactonic acidity, total acidity, pH, hydroxymethylfurfural, electrical conductivity,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apiculture; Income source; Legislation; Meliponiculture; Physicochemical profile; Stingless bees.
Ano: 2018 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122018000200201
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Sanitary quality of honey produced by Apis mellifera and angustula Tetragonisca and the need for regulatory standard Sci. Agrar.Paran. / SAP
David, Claudiane Santos; Nogueira, Vânia Rodrigues; Ronqui, Ludimilla; Lisboa, Francisco Tarcísio; Oliveira, Débora Francielly de.
This work aimed to evaluate and compare the microbiological quality of honey samples of the species Apis mellifera and Tetragonisca angustula collected by different methods, in urban and rural areas of Ariquemes and Alto Paraíso municipalities in the state of Rondônia. For this were performed analysis for counto coliforms at 35°C and 45°C, molds and yeasts and the presence of Salmonella sp. Two samples presented total coliform, having been confirmed the presence of thermotolerant coliforms in one. It was not observed the presence of Salmonella sp. in this study while the count for yeast and molds was <3.0 CFU.g-1 for all samples. The discussion occurred by comparing the data obtained by other authors, who analyzed their results by comparing with the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stingless bees; Legislation; Honey; Quality..
Ano: 2017 URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/13628
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Karyotype characterization of Trigona fulviventris Guérin, 1835 (Hymenoptera, Meliponini) by C banding and fluorochrome staining: report of a new chromosome number in the genus Genet. Mol. Biol.
Domingues,Alayne Magalhães Trindade; Waldschmidt,Ana Maria; Andrade,Sintia Emmanuelle; Andrade-Souza,Vanderly; Alves,Rogério Marco de Oliveira; Silva Junior,Juvenal Cordeiro da; Costa,Marco Antônio.
Although many species of the genus Trigona have been taxonomically described, cytogenetic studies of these species are still rare. The aim of the present study was to obtain cytogenetic data by conventional staining, C banding and fluorochrome staining for the karyotype characterization of the species Trigona fulviventris. Cytogenetic analysis revealed that this species possesses a diploid chromosome number of 2n = 32, different from most other species of this genus studied so far. This variation was probably due to the centric fusion in a higher numbered ancestral karyotype, this fusion producing the large metacentric chromosome pair and the lower chromosome number observed in Trigona fulviventris. Heterochromatin was detected in the pericentromeric...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Cytogenetics; Heterochromatin; Evolution; Stingless bees.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300009
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Aprendendo a conviver com as abelhas-arapuás em sistemas agrícolas. Infoteca-e
DRUMOND, P. M.; RIBEIRO, M. de F.; KIILL, L. H. P.; SANTOS, R. S..
Apesar dos inúmeros benefícios associados às abelhas-sem-ferrão, algumas espécies são consideradas pragas agrícolas (como as arapuás, por exemplo), por danificarem caules, flores, folhas e/ou frutos, facilitando a entrada de organismos causadores de doenças, prejudicando o desenvolvimento das plantas, bem como reduzindo a quantidade e o valor comercial dos frutos. Esta publicação reúne algumas medidas que podem auxiliar os produtores rurais a minimizar os impactos negativos causados pelas abelhas-arapuás e a conviver com esse grupo de insetos, o qual desempenha importante papel no ecossistema.
Tipo: Documentos (INFOTECA-E) Palavras-chave: Abejas sin aguijón; Abejas melíferas; Arapuá; Abelha; Irapuã; Trigona Spinipes; Manejo; Apicultura; Transferência de Tecnologia; Stingless bees; Honey bees; Apidae; Apiculture; Technology transfer.
Ano: 2019 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1113555
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Pollen analysis of the post-emergence residue of Melipona (Melikerria) interrupta Latreille (Hymenoptera: Apidae) bred in the central Amazon region Acta Botanica
Ferreira,Marcos Gonçalves; Absy,Maria Lúcia.
We applied an "adapted" protocol for collecting and processing pollen grains in the pollen analysis of the post-emergence residue of Melipona (Melikerria) interrupta Latreille. The study was conducted at the Sant'Ana honey farm, located on the banks of the Solimões River, in the municipality of Manacapuru, in the state of Amazonas, Brazil, where a colony was monitored in October and November 2010. From that colony, 10 samples of post-emergence residue were collected. Unlike in the acetolysis method, there was no need to expose pollen grains to an acidic medium, because pollen loses its content during the larval digestive process. We identified 32 pollen types, from 19 botanical families, plus three undetermined pollen types. The most representative family...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stingless bees; Palynology; Pollen; Floral resources.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000400009
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Labor division in Melipona compressipes fasciculata Smith (Hymenoptera: Apidae: Meliponinae) Anais da SEB
Giannini,Katia M..
Labor division in the stingless bee Melipona compressipes fasciculata Smith was analyzed and compared with that of other eusocial bees Meliponini and Trigonini. The results showed a sequence of activities which was similar to that of other stingless bees already studied, such as: grooming; cerumen work; cell construction; cleaning; the colony; provisioning and oviposition process; ventilation; enclosure gap; nectar reception; and foraging.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Meliponini; Stingless bees; Division of labor; Eusocial bees.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591997000100020
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Flight activity and colony strength in the stingless bee Melipona bicolor bicolor (Apidae, Meliponinae) BJB
HILÁRIO,S. D.; IMPERATRIZ-FONSECA,V. L.; KLEINERT,A. de M. P..
Flight activity of Melipona bicolor bicolor, coming from Cunha (23º05'S, 44º55'W), Atlantic Forest, was studied in ten colonies, and in two periods: from July to September 1993 and from August to September 1995. The colonies were grouped in weak, medium and strong, according to the diameter of the combs, which can provide a good idea of the number of cells built. 855 observations were accomplished for 5 minutes, every half-hour, from 8 to 18 hours. The total number of bees that entered and left the hive and the number of bees that arrived with mud, pollen and resin, besides the number that went out with debris in that period were counted. It was also registered the temperature and the relative humidity of the air. The total external activity, as well as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stingless bees; Flight activity; Climate conditions; Resource collection.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082000000200014
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Responses to climatic factors by foragers of Plebeia pugnax Moure (in litt.) (Apidae, Meliponinae) BJB
HILÁRIO,S. D.; IMPERATRIZ-FONSECA,V. L.; KLEINERT,A. de M. P..
Flight activity of Plebeia pugnax Moure (in litt.) was studied in six colonies coming from Cunha, SP, from July to October 1994. Twice a week, from 8:00 a.m. to 6:00 p.m., for 5 minutes every half-hour, all the bees entering and leaving the hives were counted. Six hundred counts were made and the materials that foragers carried were recorded. Data were analysed in relation to temperature, relative humidity, light intensity and day time. Foragers' flight activity was relatively constant in a wide range of temperature, from 22°C to 34°C. The minimum temperature for the beginning of flight activity was 14°C. Effective flight activity (when foragers of all colonies were leaving the hives) occurred at 15°C. These bees also flew within a wide range of relative...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Flight activity; Stingless bees; Climatic factors.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082001000200003
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Can climate shape flight activity patterns of Plebeia remota Hymenoptera, Apidae)? Iheringia, Sér. Zool.
Hilário,Sergio D.; Ribeiro,Márcia de F.; Imperatriz-Fonseca,Vera L..
Flight activity of foragers of four colonies of Plebeia remota (Holmberg, 1903) was registered from December 1998 to December 1999, using an automated system (photocells and PLC system). The colonies originated from two different regions: Cunha, state of São Paulo, and Prudentópolis, state of Paraná, Brazil. Flight activity was influenced by different climatic factors in each season. In the summer, the intensity of the correlations between flight activity and climatic factors was smaller than in the other seasons. During the autumn and winter, solar radiation was the factor that most influenced flight activity, while in the spring, this activity was influenced mainly by temperature. Except in the summer, the various climatic factors similarly influenced...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stingless bees; Foraging; Climatic factors; Seasonality; Geographic distribution.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212012000300004
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Melipona garbage bees release their cargo according to a Gaussian distribution BJB
KERR,A. S.; KERR,W. E..
Worker bees of Melipona compressipes and Melipona scutellaris (Meliponinae, Apidae) colonies, involved in carrying garbage to the outside of the nest, dump each individual load cargo of cocoon pellets (cocoon + larval feces + remaining wax). For Melipona scutellaris they weight a mean of 27.8 mg, and are dropped at a distance of 1 to 45 m and between 1 and 35 m for M. compressipes, according to a Gaussian Distribution. In the case of M. scutellaris the expression is: <img src="http:/img/fbpe/rbbio/v59n1/a15e1.gif"> where, N(x) = number of pellets; Exp = e elevated to the expression in brackets; x = distance in meters; the linear term (bx) describes accidental cargo slipping. The constants for the observed data are: b = 0.69; A = 1363; sigma = 2.16 m;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stingless bees; Melipona scutellaris; Melipona compressipes; Garbage bees; Gaussian Distribution; Garbage release.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71081999000100015
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Comparative study on the use of specific and heterologous microsatellite primers in the stingless bees Melipona rufiventris and M. mondury (Hymenoptera, Apidae) Genet. Mol. Biol.
Lopes,Denilce Meneses; Campos,Lúcio Antônio de Oliveira; Salomão,Tânia Maria Fernandes; Tavares,Mara Garcia.
Due to their high degree of polymorphism, microsatellites are considered useful tools for studying population genetics. Nevertheless, studies of genetic diversity in stingless bees by means of these primers have revealed a low level of polymorphism, possibly the consequence of the heterologous primers used, since in most cases these were not specifically designed for the species under consideration. Herein we compared the number of polymorphic loci and alleles per locus, as well as observed heterozygosity in Melipona rufiventris and M. mondury populations, using specific and heterologous primers. The use of specific primers placed in evidence the greater frequency of polymorphic loci and alleles per locus, besides an expressive increase in observed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Melipona; Stingless bees; Microsatellite; Transferability.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200030
Registros recuperados: 42
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